检测猪流行性腹泻病毒S1蛋白抗体的间接ELISA方法

doi:10.11843/j.issn.0366-6964.2017.06.013

Abstract

Abstract:

The present study aimed to develop an indirect ELISA for detecting antibody to porcine epidemic diarrhea virus (PEDV) spike (S) protein and analyze the dynamic of maternal special antibodies in the serum from neonatal piglets born by sows immunized with PEDV inactivated vaccine. An indirect ELISA was established using recombinant partial PEDV spike (S1) protein as detecting antigen. And anti-PEDV antibodies in the immunized sows' colostrum, sera from sows at the delivery day and from the neonatal piglets at 1, 7, 14, 21, 28 and 35 days were detected with the established ELISA. Results suggested that the ELISA method could be used to detect the special antibodies against PEDV without cross reactions with those antibodies to classical swine fever virus, porcine circovirus type 2, porcine reproductive and respiratory syndrome virus, pseudorabies virus, porcine transmissible gastroenteritis virus, and porcine rotavirus. The sensitivity of ELISA was higher than that of the immunoperoxidase monolayer assay (IPMA) and the coincidence rate was 97.14% when the same serum samples were detected using the ELISA and IPMA. The coefficients of variation (CV) of repeated in-batch and batch-to-batch tests of ELISA were less than 10%. Positive rate of anti-PEDV antibody in the colostrum and serum of all immunized sows was 100% with a higher average antibody level at the delivery day in the serum than that in the colostrum, and the specific antibody positive rate of one-day old neonatal piglets was 50% with a lower average antibody level in serum similar to that in the colostrum. And anti-PEDV antibodies in all of the piglet serum turned negative after day 7. The present study shows that PEDV antibodies could be detected using the established ELISA. The low positive rate and levels of maternal special antibodies are present in the serum of neonatal piglets born by the sows immunized once with PEDV inactivated vaccine before farrowing.

CLC Number:

S852.659.6

YAO Zuo-jun, HAO Da-ren, BAI Yun, YAN Guo-hua, WANG Hai-min, SONG Qin-ye, LI Tan-qing. Indirect ELISA for Detecting Antibody to Porcine Epidemic Diarrhea Virus S1 Protein[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1085-1091.

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Indirect ELISA for Detecting Antibody to Porcine Epidemic Diarrhea Virus S1 Protein

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